Reviews of alkaline proteases from microbial origin as an essential tool for a wide array of industrial applications

Proteolytic enzymes are present in all living organisms and aid cell growth and differentiation. Proteases are hydrolytic enzymes that act as biocatalysts for the breakdown of proteins into smaller peptides and amino acids. Microorganisms have proven to be a competent source and economical alkaline. Protease enzymes can produce a continuous and constant supply of the desired product. Alkaline proteases have a wide range of uses in various industrial sectors, particularly in the detergent and leather industries. However, their application in food has not been used much. This overview summarizes all alkaline proteases application reports in different sectors with the main insight into food applications. The effect of various physicochemical parameters on the alkaline protease is discussed. Various sources of isolation and optimal pH and temperature values ​​of bacterial and fungal species that produce alkaline protease are also reported.


Enzymes are vital biocatalysts that catalyze almost all biological processes.

1. Since ancient times, enzymes have been used in the production of various foods such as beer, wine, vinegar, cheese, and sourdough as well as in the production of raw materials. B. Leather, flax, and indigo.
2. Biocatalysis has become a necessary tool in the industrial production of active pharmaceuticals, agrochemical and pharmaceutical intermediates, bulk chemicals, and food ingredients.
3. Although enzymes were not originally used in pure form, fermentation processes produced pure enzymes which were eventually developed on a large scale from a particular strain. Microorganisms have made a significant contribution to industrial biology.
4. Microbial enzymes play an important role as metabolic catalysts and are therefore used in various industrial applications. They are hydrolytic and are used to break down various natural substances.

Protease remains the dominant type of enzyme due to its widespread use in the dairy and detergent industries. Proteases are very important enzymes and account for more than 60% of the total enzyme turnover worldwide.5 These enzymes can be divided into two large groups: endopeptidases, which cleave internal peptide bonds, and exopeptidases, which cleave internal peptide bonds.